NOT KNOWN FACTUAL STATEMENTS ABOUT 2-FDCK HCL

The lab says fixation will bring about the dyes to leak in the cells. I think the assumption is always that fixation may destruction cell membranes. I did locate a paper which describes fixation making use of methanol or acetic acid, immediately after staining having a DCFDA derivative. DCFDA could be the reagent that stains ROS during the kit ab11